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Name: Leung Kin Man University number: 2009109720 Date of try out: 25-2-2010 BIOL1133: biologic Science Laboratory Course Experiment 4: Expression of humanitys secretin receptor concretion protein in E coli. Aim: The suggest of the experiment is to express human secretin receptor fusion protein in E coli by conducting SDS-PAGE gel ionophoresis and estimate the molecular size of that protein. Abstract: In this experiment, the human secretin receptor fusion protein was expressed exploitation the SDS-PAGE technique. The pre-induced and the sample after IPTG induction was use to extract the human secretin receptor protein by use denaturing buffer zone and elution buffer. The molecular weight of the protein feces also be estimated by comparing the bands on the protein ladder. access: Massive production of active protein is usually done by recombinant deoxyribonucleic acid technology. E coli. was employ as vector for debut and expression of fusion prote in. E coli. is a germ-negative bacteria with copy membrane layer and gamin plasmid. In this experiment, the N-terminal extracellular mankind of the human secretin receptor in E coli. was expressed using IPTG inducible system.

Secretin is the key hormone that prudent for the regulation of gastrointestinal engage by regulating the secretion of bicarbonate and enzyme from the exocrine pancreas. This suffer help to neutralize stomachic acid (hydrochloric acid) and hence confine an optimum pH care for for intestinal digestion. The action of secretin is chiefly controlled by high-affinity membrane receptors distr ibuted in our body and it is tissue-specific! . The N-terminal secret writing orbit of the extracellular domain of the receptor protein is obtained by conducting polymerase chain chemical reaction (PCR) mutagenesis method. This technique was achieved by using specific desoxyribonucleic acid primers and DNA strand as a template to start the PCR reaction. The coding sequence was introduced into the bacterial DNA as a toter of the coding sequence. Upon IPTG...If you want to posture a full essay, hunting lodge it on our website: BestEssayCheap.com

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